Nanobore ESI-MS analysis has proven its functionality as a standard-free, semi-quantitative vehicle for parent-molecule and associated metabolite analysis1.Prior investigations have revealed this equimolar response can occur over a limited flow-rate interval. Further research has affirmed the capacity of silicone-chip-based nanobore electrospray mass spectrometry to provide extended dynamic ranges in absolute quantitative analysis2,3. A similar lineardynamic-range behavior for internal-standard (IS)-spiked solutions containing verapamil and testosterone analytes in a canine-plasma matrix using metallic-coated static nanospray emitters of various tip IDs was observed. Chemical structures for both analytes and their respective internal standards are illustrated in Figures 1 and 2.

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